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2 edition of Metabolism of isolated barley allurone layers. found in the catalog.

Metabolism of isolated barley allurone layers.

Vincent James Clutterbuck

Metabolism of isolated barley allurone layers.

by Vincent James Clutterbuck

  • 238 Want to read
  • 17 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - Univ. of Birmingham, Dept of Biochemistry.

ID Numbers
Open LibraryOL20283143M

GA and ABA Regulate PCD in Barley Aleurone Cells. Freshly isolated aleurone layers (Chrispeels and Varner, ) and protoplasts (Bush et al., ) of cv Himalaya barley grain synthesize α-amylase in the absence of added GA because small amounts of endogenous GAs are present in the endosperm of the mature grain (Green et al., ).To eliminate responses to endogenous GAs, we isolated. Response of Barley Aleurone Layers to Abscisic Acid Article (PDF Available) in Plant physiology 57(2) March with 9 Reads How we measure 'reads'.

Barley (Hordeum vulgare) is one of the major food sources for humans and forage sources for animal livestock. The average grain protein content (GPC) of barley ranges between 8 and 12%. Barley hordeins (i.e., prolamins) account for more than 50% of GPC in mature seeds and are important for both grain and flour quality. Barley endosperm is structured into three distinct cell layers: the starchy. The Metabolism of Soluble Nucleotides in Wheat Aleurone Layers Treated with Gibberellic Acid Article (PDF Available) in Plant physiology 49(3) April with 17 Reads How we measure 'reads'.

Aleurone layers of Hordeum vulgare, cv. `Himalaya' took up [14C]-abscisic acid (ABA) when incubated for various times. Radioactivity accumulated with time in a low speed, DNA-containing pellet accounting for to % of the radioactivity recovered in subcellular fractions at 18 hours. Thin layer chromatography of ethanolic or methanolic extracts of the cytosol, which contained greater than. Metabolism of hydrogen peroxide during reserve mobilization and programmed cell death of barley (Hordeum vulgare L.) aleurone layer cells Article in Free Radical Biology and Medicine 35(10)


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Metabolism of isolated barley allurone layers by Vincent James Clutterbuck Download PDF EPUB FB2

Chandra GR, Varner JE. Gibberellic acid-controlled metabolism of RNA in aleurone cells of barley. Biochim Biophys Acta. Dec 9; (4)– Zwar JA, Jacobsen JV. A Correlation between a Ribonucleic Acid Fraction Selectively Labeled in the Presence of Gibberellic Acid and Amylase Synthesis in Barley Aleurone by: Active polyamine metabolism occurs in the aleurone cells of deembryonate barley half seeds during imbibition.

The aleurone layers isolated from fully imbibed half seeds contain about nanomoles of Put, nanomoles of Spd, and nanomoles of Spm as free form per gram tissue dry weight while the levels of Cad and Agm are relatively by: Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers.

Plant Physiol. Mar; 42 (3)– [PMC free article] Chrispeels MJ, Varner JE. Hormonal control of enzyme synthesis: on the mode of action of gibberellic Acid and abscisin in aleurone layers of by:   Aleurone layers taken at different stages from intact germinating and germinated barley grains exhibited a similar pattern in their capacity for H 2 O 2-metabolism, albeit over a longer time-course.

A peak in H 2 O 2 scavenging activity occurs after 8–9 d following the start of grain imbibition and declines thereafter, particularly between 9 Cited by:   Ear. Cell Biol., 52 () 24 R.L. Jones, The isolation of endoplasmic reticulum from barley Metabolism of isolated barley allurone layers.

book layers. Planta, () 25 M.J. Chrispeels and J.E. Varner, Gibberellic acid enhanced synthesis and release of a-amylase and ribonuclease by isolated barley aleurone by: Summary. When aleurone layers were treated with labeled gibberellin A 1 (3 H-GA 1), gibberellin A 5 (3 H-GA 5) and the methyl ester of 3 H-GA 5 (3 H-GA 5-ME), radioactivity was accumulated by the tissue for a period of 20–30 this time, radioactivity was released into the medium.

Concomitantly, ribonuclease was also liberated by the tissue. The regulation of metabolism of abscisic acid has been investigated in the isolated aleurone layers of barley (Hordeum vulgare L.) seeds.

The rate of conversion of abscisic acid to phaseic acid is enhanced by two to five-fold when the tissue is pretreated with 10 −5 M of this hormone. Gibberellic Acid Aleurone Layer Nucleic Acid Metabolism Plant Growth Substance Barley Endosperm These keywords were added by machine and not by the authors.

This process is experimental and the keywords may be updated as the learning algorithm improves. Isolated aleurone layers, which consist of only one type of cells, can respond to exogenous gibberellic acid (GA 3) (one of the gibberellins) in a fashion comparable to what happens during seed the presence of GA 3, the activity of α-amylase increases with a lag period of 4–8 hr (Fig.

11) (Chispeels and Varner, ).After 12 hr of hormone treatment, there is a linear and. Incorporation of L-[UC] arginine or L-[UC] ornithine into putrescine (Put), spermidine (Spd) and spermine (Spm) in embryonectomized barley seeds (Hordeum vulgare L. Himalaya) was studied following imbition with methylglyoxal-bis (guanylhydrazone) (MGBG) and abscisic acid (ABA).

Both radiolabeled amino acids were incorporated into the amines as a result of active polyamine. The patterns of protein synthesis in barley aleurone layers treated with gibberellic acid (GA3) and abscisic acid (ABA) are compared with the patterns observed in wheat germ in vitro translation assays directed by RNA isolated from similarly treated layers.

When used alone, GA3 and ABA both induce the formation of new translatable mRNAs and cause new proteins to be synthesized. Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo -[H]inositol or [32Pi], extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques.

Three phospholipids were found to incorporate both myo -[H]inositol and [32Pi]—phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol.

Abstract. Barley (c.v. Himalaya) aleurone layers were incubated in [3 H]gibberellin A 1 (GA 1) at low 3 and 4 C, 3 H-activity was steadily accumulated in aleurone layers, and this accumulation was correlated with significant [3 H]GA 1 metabolism. At 1 and C, metabolism could not be detected, and at these temperatures aleurone layers equilibrated with the [3 H]GA 1.

The ABA enhancement of its own metabolism in barley aleurone layers is detectable with a pretreatment of ABA ranging from 10 −3 to 10 −4 molar. However, the rate of synthesis of poly(A)-RNA is enhanced by gibberellic acid. This enhancement begins within hr of addition of the hormone and reaches a maximum, which is about % over the control, hr after addition of the hormone.

Cordycepin inhibits total RNA as well as poly(A)-RNA synthesis in barley aleurone layers. Aleurone layers, according to the estimations of Newman and Briggs contain approximately 2/3 of triacylglycerols of barley grain, with their concentration close to 15% of fresh weight of this tissue; the remaining 1/3 is localized in the scutellum in which lipid concentration can be even higher, up to 40% of fresh weight in maize scutellum.

In the barley (Hordeum vulgare) aleurone layer, the phytohormone gibberellic acid (GA) induces germination-associated metabolism, enzyme secretion and subsequent PCD. Abscisic acid (ABA) counteracts GA, and in the presence of both phytohormones germination-related events of the aleurone layer are delayed, while ABA suppresses PCD entirely in.

A Correlation between a Ribonucleic Acid Fraction Selectively Labeled in the Presence of Gibberellic Acid and Amylase Synthesis in Barley Aleurone Layers. Plant Physiol. Jun; 49 (6)– Van Onckelen HA, Verbeek R, Khan AA. Relationship of ribonucleic Acid metabolism in embryo and aleurone to alpha-amylase synthesis in barley.

The induction of α-amylase synthesis in endosperm halves of barley seeds by gibberellic acid (GA) was discovered by Paleg () and by Yomo (). Later Paleg () and Varner () showed that the site of enzyme production was the aleurone layer and that the GA effect could be obtained in aleurone layers isolated from the starchy endosperm.

Jacobsen JV, Scandalios JG, Varner JE. Multiple forms of amylase induced by gibberellic acid in isolated barley aleurone layers. Plant Physiol. Apr; 45 (4)– [PMC free article] Jones KC. Similarities between gibberellins and related compounds in inducing Acid phosphatase and reducing sugar release from barley endosperm.

The secretion of alpha-amylase from single isolated (Hordeum vulgare L. cv Himalaya) aleurone layers was studied in an automated flow-through apparatus. Barley (Hordeum vulgare L. cv. Himalaya) aleurone layers and aleurone protoplasts were isolated from grain of the harvest obtained from the Department of Agronomy, Washington State University, Pullman, WA, USA.

Aleurone layers were prepared as described previously (Fath et al., ) and incubated in media containing 20 mM CaCl 2 and 5 μM.The barley aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of the effect of added signalling molecules in an isolated system.

These properties have led to its use as a model system for the study of plant signalling and germination.